UNIT 4.27 Measurement of In Vitro Cytochrome P450 2B6 Activity
Published Online: 1 FEB 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Walsky, R. L. and Obach, R. S. 2009. Measurement of In Vitro Cytochrome P450 2B6 Activity. Current Protocols in Toxicology. 39:4.27:4.27.1–4.27.11.
- Published Online: 1 FEB 2009
- Published Print: FEB 2009
Cytochrome P450 2B6 (CYP2B6) is responsible for the metabolism of a number of therapeutically relevant drugs and environmental chemicals. While not as frequently involved in xenobiotic metabolism as other more commonly studied P450 enzymes such as CYP3A, CYP2D6, or CYP2C9, its importance is becoming increasingly realized. Bupropion hydroxylase activity is an excellent indicator of in vitro CYP2B6 activity, and the metabolite formed is easily measured by HPLC/MS/MS. This assay allows the use of very low concentrations of human liver microsomes or recombinant enzyme, and it represents a selective, sensitive approach to assessing in vitro CYP2B6 activity, with minimal sample preparation. Curr. Protoc. Toxicol. 39:4.27.1-4.27.11. © 2009 by John Wiley & Sons, Inc.