UNIT 4.28 Azoreductase in Staphylococcus aureus

  1. Wen Zou,
  2. Carl E. Cerniglia,
  3. Huizhong Chen

Published Online: 1 AUG 2009

DOI: 10.1002/0471140856.tx0428s41

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Zou, W., Cerniglia, C. E. and Chen, H. 2009. Azoreductase in Staphylococcus aureus. Current Protocols in Toxicology. 41:4.28:4.28.1–4.28.9.

Author Information

  1. National Center for Toxicological Research/FDA, Jefferson, Arkansas

Publication History

  1. Published Online: 1 AUG 2009
  2. Published Print: AUG 2009


Azoreductase(s) catalyze a NAD(P)H-dependent reaction in bacteria to metabolize azo dyes to colorless aromatic amines. Azoreductases from bacteria represent a novel family of enzymes with little similarity to other reductases. This unit will describe the current methods for measuring azoreductase from Staphylococcus aureus, which has been suggested to serve as a model strain to study the azo dye degradation by human skin microflora. Curr. Protoc. Toxicol. 41:4.28.1-4.28.9. © 2009 by John Wiley & Sons, Inc.


  • azoreductase;
  • azo dye;
  • Staphylococcus aureus;
  • enzyme activity;
  • metabolites