UNIT 4.33 Analysis of Arsenical Metabolites in Biological Samples

  1. Araceli Hernandez-Zavala1,
  2. Zuzana Drobna2,
  3. Miroslav Styblo1,2,
  4. David J. Thomas3

Published Online: 1 NOV 2009

DOI: 10.1002/0471140856.tx0433s42

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Hernandez-Zavala, A., Drobna, Z., Styblo, M. and Thomas, D. J. 2009. Analysis of Arsenical Metabolites in Biological Samples. Current Protocols in Toxicology. 42:4.33:4.33.1–4.33.17.

Author Information

  1. 1

    Center for Environmental Medicine, Asthma, and Lung Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina

  2. 2

    Department of Nutrition, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina

  3. 3

    Pharmacokinetics Branch, Experimental Toxicology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina

Publication History

  1. Published Online: 1 NOV 2009
  2. Published Print: NOV 2009


Quantitation of iAs and its methylated metabolites in biological samples provides dosimetric information needed to understand dose-response relations. Here, methods are described for separation of inorganic and mono-, di-, and trimethylated arsenicals by thin-layer chromatography. This method has been extensively used to track the metabolism of the radionuclide [73As] in a variety of in vitro assay systems. In addition, a hydride generation-cryotrapping-gas chromatography-atomic absorption spectrometric method is described for the quantitation of arsenicals in biological samples. This method uses pH-selective hydride generation to differentiate among arsenicals containing trivalent or pentavalent arsenic. Curr. Protoc. Toxicol. 42:4.33.1-4.33.17. © 2009 by John Wiley & Sons, Inc.


  • arsenic;
  • methylated arsenicals;
  • [73As];
  • thin-layer chromatography;
  • hydride generation-cryotrapping-gas chromatography-atomic absorption spectrometry;
  • pH-selective hydride generation;
  • arsenic oxidation state