UNIT 4.33 Analysis of Arsenical Metabolites in Biological Samples
Published Online: 1 NOV 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Hernandez-Zavala, A., Drobna, Z., Styblo, M. and Thomas, D. J. 2009. Analysis of Arsenical Metabolites in Biological Samples. Current Protocols in Toxicology. 42:4.33:4.33.1–4.33.17.
- Published Online: 1 NOV 2009
- Published Print: NOV 2009
Quantitation of iAs and its methylated metabolites in biological samples provides dosimetric information needed to understand dose-response relations. Here, methods are described for separation of inorganic and mono-, di-, and trimethylated arsenicals by thin-layer chromatography. This method has been extensively used to track the metabolism of the radionuclide [73As] in a variety of in vitro assay systems. In addition, a hydride generation-cryotrapping-gas chromatography-atomic absorption spectrometric method is described for the quantitation of arsenicals in biological samples. This method uses pH-selective hydride generation to differentiate among arsenicals containing trivalent or pentavalent arsenic. Curr. Protoc. Toxicol. 42:4.33.1-4.33.17. © 2009 by John Wiley & Sons, Inc.
- methylated arsenicals;
- thin-layer chromatography;
- hydride generation-cryotrapping-gas chromatography-atomic absorption spectrometry;
- pH-selective hydride generation;
- arsenic oxidation state