Unit

UNIT 4.39 Simultaneous, Untargeted Metabolic Profiling of Polar and Nonpolar Metabolites by LC-Q-TOF Mass Spectrometry

  1. Jay S. Kirkwood1,
  2. Claudia Maier2,
  3. Jan F. Stevens1

Published Online: 1 MAY 2013

DOI: 10.1002/0471140856.tx0439s56

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Kirkwood, J. S., Maier, C. and Stevens, J. F. 2013. Simultaneous, Untargeted Metabolic Profiling of Polar and Nonpolar Metabolites by LC-Q-TOF Mass Spectrometry. Current Protocols in Toxicology. 56:4.39:4.39.1–4.39.12.

Author Information

  1. 1

    Linus Pauling Institute and Department of Pharmaceutical Sciences, Oregon State University, Corvallis, Oregon

  2. 2

    Department of Chemistry, Oregon State University, Corvallis, Oregon

Publication History

  1. Published Online: 1 MAY 2013

Abstract

At its most ambitious, untargeted metabolomics aims to characterize and quantify all of the metabolites in a given system. Metabolites are often present at a broad range of concentrations and possess diverse physical properties complicating this task. Performing multiple sample extractions, concentrating sample extracts, and using several separation and detection methods are common strategies to overcome these challenges but require a great amount of resources. This protocol describes the untargeted, metabolic profiling of polar and nonpolar metabolites with a single extraction and using a single analytical platform. Curr. Protoc. Toxicol. 56:4.39.1-4.39.12. © 2013 by John Wiley & Sons, Inc.

Keywords:

  • untargeted metabolomics;
  • LC-MS/MS;
  • hypothesis generation