UNIT 5.1 Measurement of Bioavailability: Measurement of Absorption Through Skin In Vitro
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Hotchkiss, S. A. 2001. Measurement of Bioavailability: Measurement of Absorption Through Skin In Vitro. Current Protocols in Toxicology. 00:5.1:5.1.1–5.1.14.
- Published Online: 1 MAY 2001
- Published Print: MAY 1999
New therapeutic compounds intended for use on the skin or for delivery through application to the skin and agrochemicals, whose use may result in skin exposure, must be tested for bioavailability as the result of absorption. This unit contains a protocol for measuring skin absorption in vitro using the diffusion cell skin absorption method (SAM), which can be used to measure percutaneous absorption after topical application. Usually a radiolabeled compound is used, but if a suitable specific assay is available, nonradioactive compounds may be tested. The procedure is applicable to skin from a variety of species.