Unit

UNIT 5.1 Measurement of Bioavailability: Measurement of Absorption Through Skin In Vitro

  1. Sharon A.M. Hotchkiss

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.tx0501s00

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Hotchkiss, S. A. 2001. Measurement of Bioavailability: Measurement of Absorption Through Skin In Vitro. Current Protocols in Toxicology. 00:5.1:5.1.1–5.1.14.

Author Information

  1. Imperial College School of Medicine, London, United Kingdom

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAY 1999

Abstract

New therapeutic compounds intended for use on the skin or for delivery through application to the skin and agrochemicals, whose use may result in skin exposure, must be tested for bioavailability as the result of absorption. This unit contains a protocol for measuring skin absorption in vitro using the diffusion cell skin absorption method (SAM), which can be used to measure percutaneous absorption after topical application. Usually a radiolabeled compound is used, but if a suitable specific assay is available, nonradioactive compounds may be tested. The procedure is applicable to skin from a variety of species.