UNIT 5.2 Measurement of Bioavailability: Measuring Absorption Through Skin In Vivo in Rats and Humans
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Bounds, S. V. and Hawkins, D. R. 2001. Measurement of Bioavailability: Measuring Absorption Through Skin In Vivo in Rats and Humans. Current Protocols in Toxicology. 00:5.2:5.2.1–5.2.10.
- Published Online: 1 MAY 2001
- Published Print: MAY 1999
Absorption through the skin is a route of exposure to a wide variety of therapeutic and/or environmental compounds. In vivo assays are advantageous in that they retain intact epidermal and dermal structures and thus reflect a more normal situation. The test compound is applied to the skin in a protected area for a specified period of time. At the end of the incubation, skin, tissues, and excreta are assessed for the presence of the test compound. The assay can also be performed with volatile compounds. When performed using laboratory animals, it is possible to assess the distribution of the compound throughout the body. If human volunteers are studied such analysis is limited to the skin, blood, and excreta.