UNIT 5.6 Toxicant Transport by P-Glycoprotein
Published Online: 1 MAY 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Bain, L. J. 2003. Toxicant Transport by P-Glycoprotein. Current Protocols in Toxicology. 15:5.6:5.6.1–5.6.13.
- Published Online: 1 MAY 2003
- Published Print: FEB 2003
Toxicant Transport by P-Glycoprotein (Lisa J. Bain, University of Texas at El Paso, El Paso, Texas).This unit describes several different methods of measuring active transport of toxicants by P-glycoprotein uptake of a radiolabeled toxicant, uptake of an unlabeled toxicant with detection by HPLC or GC, uptake of radiolabeled toxicant in membrane vesicles; movement of radiolabeled toxicant from the basal to apical compartment using polarized epithelial cells grown in Transwell culture. P-glygoprotein is an important protein for limiting entry into the brain through the blood-bain barrier. It also alters the rates of uptake and elimination.