UNIT 5.7 Collection of Bile and Urine Samples for Determining the Urinary and Hepatobiliary Disposition of Xenobiotics in Mice
Published Online: 1 JAN 2005
Copyright © 2004 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Manautou, J. E. and Chen, C. 2005. Collection of Bile and Urine Samples for Determining the Urinary and Hepatobiliary Disposition of Xenobiotics in Mice. Current Protocols in Toxicology. 22:5.7:5.7.1–5.7.7.
- Published Online: 1 JAN 2005
- Published Print: NOV 2004
One of the most simple and informative method commonly used by researchers in the field of xenobiotics metabolism and dispostion to investigate the fate of chemicals in living organisms involves identification and/or quantitation of metabolites in excreta such as urine and bile. This method can be performed on dogs and larger rodents such as rats and hamsters rather easily. However, many investigators find it difficult to collect bile samples from mice. This unit describes, in detail, how to collect urine and bile samples from a mouse for further metabolite analysis.