Unit

UNIT 5.7 Collection of Bile and Urine Samples for Determining the Urinary and Hepatobiliary Disposition of Xenobiotics in Mice

  1. José E. Manautou,
  2. Chuan Chen

Published Online: 1 JAN 2005

DOI: 10.1002/0471140856.tx0507s22

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Manautou, J. E. and Chen, C. 2005. Collection of Bile and Urine Samples for Determining the Urinary and Hepatobiliary Disposition of Xenobiotics in Mice. Current Protocols in Toxicology. 22:5.7:5.7.1–5.7.7.

Author Information

  1. University of Connecticut, Storrs, Connecticut

Publication History

  1. Published Online: 1 JAN 2005
  2. Published Print: NOV 2004

Abstract

One of the most simple and informative method commonly used by researchers in the field of xenobiotics metabolism and dispostion to investigate the fate of chemicals in living organisms involves identification and/or quantitation of metabolites in excreta such as urine and bile. This method can be performed on dogs and larger rodents such as rats and hamsters rather easily. However, many investigators find it difficult to collect bile samples from mice. This unit describes, in detail, how to collect urine and bile samples from a mouse for further metabolite analysis.