UNIT 6.2 Measurement of Glutathione and Glutathione Disulfide

  1. Debbie Mustacich

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.tx0602s00

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Mustacich, D. 2001. Measurement of Glutathione and Glutathione Disulfide. Current Protocols in Toxicology. 00:6.2:6.2.1–6.2.14.

Author Information

  1. Arizona Cancer Center, Tucson, Arizona

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAY 1999


Measurements of glutathione should include quantification of both the reduced and oxidized forms. HPLC-based assays for glutathione and other cellular thiols and disulfides utilize a variety of detection methods, including ultraviolet, fluorescence, and electrochemical methods. This unit provides rapid, sensitive, highly versatile and reproducible HPLC methods for separating and quantifying sulfur-containing amino acids and related derivatives from a variety of biological samples.