UNIT 6.2 Measurement of Glutathione and Glutathione Disulfide
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Mustacich, D. 2001. Measurement of Glutathione and Glutathione Disulfide. Current Protocols in Toxicology. 00:6.2:6.2.1–6.2.14.
- Published Online: 1 MAY 2001
- Published Print: MAY 1999
Measurements of glutathione should include quantification of both the reduced and oxidized forms. HPLC-based assays for glutathione and other cellular thiols and disulfides utilize a variety of detection methods, including ultraviolet, fluorescence, and electrochemical methods. This unit provides rapid, sensitive, highly versatile and reproducible HPLC methods for separating and quantifying sulfur-containing amino acids and related derivatives from a variety of biological samples.