UNIT 6.4 Measurement of Glutathione Transferases
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Mannervik, B. and Jemth, P. 2001. Measurement of Glutathione Transferases. Current Protocols in Toxicology. 14:6.4:6.4.1–6.4.10.
- Published Online: 1 MAY 2001
- Published Print: AUG 1999
There are multiple glutathione transferase genes, the proteins for which have different substrate specificities. The various genes are differentially expressed such that species and organs and tissues differ qualitatively and quantitatively for cytosolic and membrane-bound forms. This unit provides protocols for analysis of transferase activity in a continuous spectrophotometric assay and an assay with dichloromethane as the substrate.