Unit

UNIT 6.4 Measurement of Glutathione Transferases

  1. Bengt Mannervik,
  2. Per Jemth

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.tx0604s01

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Mannervik, B. and Jemth, P. 2001. Measurement of Glutathione Transferases. Current Protocols in Toxicology. 14:6.4:6.4.1–6.4.10.

Author Information

  1. Uppsala University, Uppsala, Sweden

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: AUG 1999

Abstract

There are multiple glutathione transferase genes, the proteins for which have different substrate specificities. The various genes are differentially expressed such that species and organs and tissues differ qualitatively and quantitatively for cytosolic and membrane-bound forms. This unit provides protocols for analysis of transferase activity in a continuous spectrophotometric assay and an assay with dichloromethane as the substrate.