UNIT 6.5 HPLC-Based Assays for Enzymes of Glutathione Biosynthesis
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
White, C. C., Krejsa, C. J., Eaton, D. L. and Kavanagh, T. J. 2001. HPLC-Based Assays for Enzymes of Glutathione Biosynthesis. Current Protocols in Toxicology. 00:6.5:6.5.1–6.5.14.
- Published Online: 1 MAY 2001
- Published Print: MAY 1999
Glutamate cysteine ligase and gluthione synthase carry out the two-step synthesis of glutathione. The fluorescent thiol-reactive compound monobromobimane is used to derivatize reaction products in an HPLC-based assay with fluorescence detection. The assay described in this unit can be adapted for tissue homogenates or cultured cells.