UNIT 6.5 HPLC-Based Assays for Enzymes of Glutathione Biosynthesis

  1. Collin C. White,
  2. Cecile J. Krejsa,
  3. David L. Eaton,
  4. Terrance J. Kavanagh

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.tx0605s00

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

White, C. C., Krejsa, C. J., Eaton, D. L. and Kavanagh, T. J. 2001. HPLC-Based Assays for Enzymes of Glutathione Biosynthesis. Current Protocols in Toxicology. 00:6.5:6.5.1–6.5.14.

Author Information

  1. University of Washington, Seattle, Washington

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAY 1999


Glutamate cysteine ligase and gluthione synthase carry out the two-step synthesis of glutathione. The fluorescent thiol-reactive compound monobromobimane is used to derivatize reaction products in an HPLC-based assay with fluorescence detection. The assay described in this unit can be adapted for tissue homogenates or cultured cells.