Unit

UNIT 6.7 Oxidant-Induced Regulation of Glutathione Synthesis

  1. Rui-Ming Liu1,
  2. Jinah Choi2,
  3. Henry J. Forman1

Published Online: 1 AUG 2001

DOI: 10.1002/0471140856.tx0607s08

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Liu, R.-M., Choi, J. and Forman, H. J. 2001. Oxidant-Induced Regulation of Glutathione Synthesis. Current Protocols in Toxicology. 8:6.7:6.7.1–6.7.21.

Author Information

  1. 1

    University Of Alabama At Birmingham, Birmingham, Alabama

  2. 2

    University Of Southern California, Los Angeles, California

Publication History

  1. Published Online: 1 AUG 2001
  2. Published Print: MAY 2001

Abstract

This unit describes protocols for characterizing the expression of two glutathione biosynthesis enzymes: gamma-glutamylcysteine synthase (GCS) and gamma-glutamyl transpeptidase (GGT) in response to oxidants. GCS catalyzes the first and rate-limiting step of glutathione synthesis, while GGT degrades extracellular glutathione (GSH) to provide the amino acids required for intracellular synthesis of GSH. Northern blot hybridization is used to quantitatively assess the mRNAs for the two enzymes in response to oxidant. Nuclear run-on is used to determine the rate of transcription.