UNIT 6.9 Coenzyme A and Coenzyme A-Glutathione Mixed Disulfide Measurements by HPLC
Published Online: 1 MAY 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Rogers, L. K. and Smith, C. V. 2003. Coenzyme A and Coenzyme A-Glutathione Mixed Disulfide Measurements by HPLC. Current Protocols in Toxicology. 15:6.9:6.9.1–6.9.8.
- Published Online: 1 MAY 2003
- Published Print: FEB 2003
This unit describes a method for measuring coenzyme A and coenzyme A-glutathione mixed disulfide in tissue homogenates obtained from tissues frozen in liquid nitrogen, homogenized in the presence of N-ethylmaleimide, and then acidified. to avoid the oxidation, reduction, and thiol exchange reactions that can occur during tissue processing and isolation of subcellular fractions. This method may provide a way to assess changes in the oxidative state of mitochondria in intact tissues.