Unit

UNIT 6.9 Coenzyme A and Coenzyme A-Glutathione Mixed Disulfide Measurements by HPLC

  1. Lynette K. Rogers,
  2. Charles V. Smith

Published Online: 1 MAY 2003

DOI: 10.1002/0471140856.tx0609s15

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Rogers, L. K. and Smith, C. V. 2003. Coenzyme A and Coenzyme A-Glutathione Mixed Disulfide Measurements by HPLC. Current Protocols in Toxicology. 15:6.9:6.9.1–6.9.8.

Author Information

  1. The Ohio State University, Children's Research Institute, Columbus, Ohio

Publication History

  1. Published Online: 1 MAY 2003
  2. Published Print: FEB 2003

Abstract

This unit describes a method for measuring coenzyme A and coenzyme A-glutathione mixed disulfide in tissue homogenates obtained from tissues frozen in liquid nitrogen, homogenized in the presence of N-ethylmaleimide, and then acidified. to avoid the oxidation, reduction, and thiol exchange reactions that can occur during tissue processing and isolation of subcellular fractions. This method may provide a way to assess changes in the oxidative state of mitochondria in intact tissues.