UNIT 7.7 Catalase Activity Assays

  1. Nandita Shangari,
  2. Peter J. O'Brien

Published Online: 1 MAR 2006

DOI: 10.1002/0471140856.tx0707s27

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Shangari, N. and O'Brien, P. J. 2006. Catalase Activity Assays. Current Protocols in Toxicology. 27:7.7:7.7.1–7.7.16.

Author Information

  1. University of Toronto, Toronto, Ontario, Canada

Publication History

  1. Published Online: 1 MAR 2006
  2. Published Print: FEB 2006


Catalase (hydrogen peroxide/hydrogen peroxide oxidoreductase) is an important cellular antioxidant enzyme that defends against oxidative stress. It is found in the peroxisomes of most aerobic cells. It serves to protect the cell from toxic effects of high concentrations of hydrogen peroxide (H2O2) by catalyzing its decomposition into molecular oxygen and water, without the production of free radicals. It is important to measure catalase levels because oxidative stress is inherent in pathological conditions such as cancer, diabetes, cataracts, atherosclerosis, neurodegenerative disease, aging, and nutritional deficiencies. This unit provides methods for catalase activity measurements.


  • catalase;
  • catalytic activity;
  • hydrogen peroxide;
  • xylenol orange