Unit

UNIT 7.8 Measurement of the Vitamin E Metabolites, Carboxyethyl Hydroxychromans (CEHCs), in Biological Samples

  1. Scott W. Leonard,
  2. Maret G. Traber

Published Online: 1 SEP 2006

DOI: 10.1002/0471140856.tx0708s29

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Leonard, S. W. and Traber, M. G. 2006. Measurement of the Vitamin E Metabolites, Carboxyethyl Hydroxychromans (CEHCs), in Biological Samples. Current Protocols in Toxicology. 29:7.8:7.8.1–7.8.12.

Author Information

  1. Linus Pauling Institute, Oregon State University, Corvallis, Oregon

Publication History

  1. Published Online: 1 SEP 2006
  2. Published Print: AUG 2006

Abstract

Metabolites of α- and γ-tocopherol, 2,5,7,8-tetramethyl-2-(2′-carboxyethyl)-6-hydroxychroman (α-CEHC) and 2,7,8-trimethyl-2-(β-carboxyethyl)-6-hydroxychroman (γ-CEHC), respectively, are produced in the liver and have been measured in biological fluids and tissue. Compared to α-tocopherol concentrations, metabolite concentrations are as much as a factor of a thousand lower, requiring extremely sensitive methodology to attain accurate measurements. This unit presents a protocol for CEHC extraction from biological samples, and describes very specific and sensitive HPLC/MS analysis.

Keywords:

  • α-CEHC;
  • γ-CEHC;
  • mass spectrometry;
  • vitamin E metabolism;
  • α-tocopherol;
  • γ-tocopherol