UNIT 7.8 Measurement of the Vitamin E Metabolites, Carboxyethyl Hydroxychromans (CEHCs), in Biological Samples
Published Online: 1 SEP 2006
Copyright © 2006 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Leonard, S. W. and Traber, M. G. 2006. Measurement of the Vitamin E Metabolites, Carboxyethyl Hydroxychromans (CEHCs), in Biological Samples. Current Protocols in Toxicology. 29:7.8:7.8.1–7.8.12.
- Published Online: 1 SEP 2006
- Published Print: AUG 2006
Metabolites of α- and γ-tocopherol, 2,5,7,8-tetramethyl-2-(2′-carboxyethyl)-6-hydroxychroman (α-CEHC) and 2,7,8-trimethyl-2-(β-carboxyethyl)-6-hydroxychroman (γ-CEHC), respectively, are produced in the liver and have been measured in biological fluids and tissue. Compared to α-tocopherol concentrations, metabolite concentrations are as much as a factor of a thousand lower, requiring extremely sensitive methodology to attain accurate measurements. This unit presents a protocol for CEHC extraction from biological samples, and describes very specific and sensitive HPLC/MS analysis.
- mass spectrometry;
- vitamin E metabolism;