UNIT 8.4 Measurement of Uroporphyrinogen Decarboxylase Activity
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Phillips, J. D. and Kushner, J. P. 2001. Measurement of Uroporphyrinogen Decarboxylase Activity. Current Protocols in Toxicology. 00:8.4:8.4.1–8.4.13.
- Published Online: 1 MAY 2001
- Published Print: MAY 1999
Uroporphyrinogen decarboxylase (UROD) catalyzes decarboxylation of the four acetate side chains of urophyrinogen to form coproporphyrinogen. Activity of UROD can be measured using an enzymatically prepared substrate or a chemically prepared one. For the former, bacterial porphobilinogen deaminase is prepared and used to prepare the porphyrinogen substrate for the enzymatic assay. Erythrocyte lysates can be used to measure hemoglobin content as an indicator of UROD activity.