UNIT 8.6 Measurement of δ-Aminolevulinate Dehydratase Activity

  1. Hiroyoshi Fujita

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.tx0806s01

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Fujita, H. 2001. Measurement of δ-Aminolevulinate Dehydratase Activity. Current Protocols in Toxicology. 1:8.6:8.6.1–8.6.11.

Author Information

  1. Hokkaido University School of Medicine, Sapporo, Japan

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: AUG 1999


Vertebrate ALAD is a cytosolic enzyme that catalyzes the second step of porphyrin formation, and it is expressed abundantly in liver and erythroid tissues as well as in other tissues. This unit describes a colorimetric assay for measuring ALAD activity in mammalian blood or tissues and the extent of reactivation of the enzyme in lead-inhibited samples.