UNIT 8.6 Measurement of δ-Aminolevulinate Dehydratase Activity
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Fujita, H. 2001. Measurement of δ-Aminolevulinate Dehydratase Activity. Current Protocols in Toxicology. 1:8.6:8.6.1–8.6.11.
- Published Online: 1 MAY 2001
- Published Print: AUG 1999
Vertebrate ALAD is a cytosolic enzyme that catalyzes the second step of porphyrin formation, and it is expressed abundantly in liver and erythroid tissues as well as in other tissues. This unit describes a colorimetric assay for measuring ALAD activity in mammalian blood or tissues and the extent of reactivation of the enzyme in lead-inhibited samples.