Unit

UNIT 8.7 Measurement of Ferrochelatase Activity

  1. Shigeru Taketani

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.tx0807s02

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Taketani, S. 2001. Measurement of Ferrochelatase Activity. Current Protocols in Toxicology. 2:8.7:8.7.1–8.7.8.

Author Information

  1. Kansai Medical University, Moriguchi, Osaka

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: NOV 1999

Abstract

Ferrochelatase is the terminal enzyme in the heme biosynthesis pathway. Under anaerobic conditions it catalyzes the insertion of ferrous iron into the protoporphyrin IX ring to form protoheme. In the absence of iron and under aerobic conditions, the enzyme will use zinc or mercury as a substitute. The assay described in this unit uses zinc under aerobic conditions and the artificial substrate mesoporphyrin to analyze the formation of zinc-mesoporphyrin by HPLC with UV or fluorescence detection.