UNIT 8.7 Measurement of Ferrochelatase Activity
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Taketani, S. 2001. Measurement of Ferrochelatase Activity. Current Protocols in Toxicology. 2:8.7:8.7.1–8.7.8.
- Published Online: 1 MAY 2001
- Published Print: NOV 1999
Ferrochelatase is the terminal enzyme in the heme biosynthesis pathway. Under anaerobic conditions it catalyzes the insertion of ferrous iron into the protoporphyrin IX ring to form protoheme. In the absence of iron and under aerobic conditions, the enzyme will use zinc or mercury as a substitute. The assay described in this unit uses zinc under aerobic conditions and the artificial substrate mesoporphyrin to analyze the formation of zinc-mesoporphyrin by HPLC with UV or fluorescence detection.