Unit

UNIT 8.8 Measurement of Erythrocyte Protoporphyrin Concentration by Double Extraction and Spectrofluorometry

  1. Patrick J. Parsons

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.tx0808s02

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Parsons, P. J. 2001. Measurement of Erythrocyte Protoporphyrin Concentration by Double Extraction and Spectrofluorometry. Current Protocols in Toxicology. 2:8.8:8.8.1–8.8.12.

Author Information

  1. Wadsworth Center, New York State Department of Health, and The University at Albany, Albany, New York

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: NOV 1999

Abstract

Quantification of the level of erythrocyte protoporphyrin can be used as a screening assay for lead exposure. In this method, porphyrins and heme compounds are extracted from whole blood using ethyl acetate, and porphyrins are then separated from heme by back-extraction with HCl solution. The extracted porphyrins are then quantified using a spectrofluorometer calibrated with protoporphyrin IX standard solutions.