UNIT 8.8 Measurement of Erythrocyte Protoporphyrin Concentration by Double Extraction and Spectrofluorometry
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Parsons, P. J. 2001. Measurement of Erythrocyte Protoporphyrin Concentration by Double Extraction and Spectrofluorometry. Current Protocols in Toxicology. 2:8.8:8.8.1–8.8.12.
- Published Online: 1 MAY 2001
- Published Print: NOV 1999
Quantification of the level of erythrocyte protoporphyrin can be used as a screening assay for lead exposure. In this method, porphyrins and heme compounds are extracted from whole blood using ethyl acetate, and porphyrins are then separated from heme by back-extraction with HCl solution. The extracted porphyrins are then quantified using a spectrofluorometer calibrated with protoporphyrin IX standard solutions.