Unit

UNIT 8.9 HPLC Methods for Analysis of Porphyrins in Biological Media

  1. James S. Woods,
  2. P. Lynne Simmonds

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.tx0809s07

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Woods, J. S. and Simmonds, P. L. 2001. HPLC Methods for Analysis of Porphyrins in Biological Media. Current Protocols in Toxicology. 7:8.9:8.9.1–8.9.17.

Author Information

  1. University of Washington, Seattle, Washington

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2001

Abstract

Changes in porphyrin concentrations in biological media may serve as biological indicators of exposure and toxicity from a wide variety of drugs and chemical agents. This unit describes procedures for quantitative extraction of porphyrins from urine, feces, blood, and biological tissues as well as their separation and analysis by HAPLY spectrofluorometrc techniques.