Unit

UNIT 9.2 Detection of Heme Oxygenase Activity by Measurement of CO

  1. Hendrik J. Vreman,
  2. David K. Stevenson

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.tx0902s00

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Vreman, H. J. and Stevenson, D. K. 2001. Detection of Heme Oxygenase Activity by Measurement of CO. Current Protocols in Toxicology. 00:9.2:9.2.1–9.2.10.

Author Information

  1. Stanford University Medical Center, Stanford, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAY 1999

Abstract

Heme oxygenase (HO) is the first and rate-limiting step in degradation of heme, and in the presence of NADPH-cytochrome P-450 reductase it produces equimolar amounts of biliverdin and CO. CO produced in a closed system can be quantified as described in this unit by gas chromatography as a measure of HO activity in tissue slices, tissue homogenates, and tissue fractions.