UNIT 9.3 Detection of Heme Oxygenase 1 and 2 Proteins and Bilirubin Formation

  1. McCoubrey William K. Jr.

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.tx0903s00

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

McCoubrey, W. K. 2001. Detection of Heme Oxygenase 1 and 2 Proteins and Bilirubin Formation. Current Protocols in Toxicology. 00:9.3:9.3.1–9.3.9.

Author Information

  1. University of Rochester, Rochester, New York

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAY 1999


It is possible to detect changes in the level of expression of the two isozymes of heme oxygenase (HO). The sequence differences allow the production of isozyme-specific antibodies that can be used in immunoblot analysis. Total HO activity in tissue microsomal fractions or extracts of bacteria transformed with an HO gene can be measured as described in this unit using a coupled assay employing biliverdin reductase, which converts the biliverdin product to bilirubin. Bilirubin is then quantified with a scanning spectrophotometer.