Unit

UNIT 9.4 Detection of Biliverdin Reductase Activity

  1. Tian-Jun Huang

Published Online: 1 NOV 2002

DOI: 10.1002/0471140856.tx0904s00

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Huang, T.-J. 2002. Detection of Biliverdin Reductase Activity. Current Protocols in Toxicology. 00:9.4:9.4.1–9.4.10.

Author Information

  1. New York Blood Center, New York, New York

Publication History

  1. Published Online: 1 NOV 2002
  2. Published Print: AUG 2002

Abstract

The conversion of biliverdin to the bile pigment bilirubin is catalyzed by biliverdin reductase, a cytosolic enzyme with two pH optima with different cofactors. The enzyme is assayed at pH 8.7 with NADPH as a cofactor and at 6.75 with NADH. The production of bilirubin is detected as described in this unit with a spectrophotometric assay. The enzyme can be qualitatively assayed by immunoblotting and changes in reductase isoform expression can be detected by two-dimensional electrophoresis.