UNIT 9.6 An HPLC Method to Detect Heme Oxygenase Activity
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Ryter, S. W. and Tyrrell, R. M. 2001. An HPLC Method to Detect Heme Oxygenase Activity. Current Protocols in Toxicology. 5:9.6.1–9.6.14.
- Published Online: 1 MAY 2001
- Published Print: AUG 2000
This unit presents a method to calculate heme oxygenase enzymatic activity from the formation of bilirubin equivalents [biliverdin-Ixalpha (BV) and bilirubin-IXalpha (BR)]. The BV and BR generated in the reaction are separated by reversed-phase HPLC and detected using visible absorbance spectroscopy. Since both metabolites of heme degradation are directly quantifiable, the assay eliminates the requirement for biliverdin reductase supplementation.