Unit

UNIT 9.6 An HPLC Method to Detect Heme Oxygenase Activity

  1. Stefan W. Ryter1,
  2. Rex M. Tyrrell2

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.tx0906s05

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Ryter, S. W. and Tyrrell, R. M. 2001. An HPLC Method to Detect Heme Oxygenase Activity. Current Protocols in Toxicology. 5:9.6:9.6.1–9.6.14.

Author Information

  1. 1

    Southern Illinois University School of Medicine, Springfield, Illinois

  2. 2

    University of Bath, Bath, United Kingdom

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: AUG 2000

Abstract

This unit presents a method to calculate heme oxygenase enzymatic activity from the formation of bilirubin equivalents [biliverdin-Ixalpha (BV) and bilirubin-IXalpha (BR)]. The BV and BR generated in the reaction are separated by reversed-phase HPLC and detected using visible absorbance spectroscopy. Since both metabolites of heme degradation are directly quantifiable, the assay eliminates the requirement for biliverdin reductase supplementation.