UNIT 9.7 Functional Analysis of the Heme Oxygenase-1 Gene Promoter
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Alam, J. 2001. Functional Analysis of the Heme Oxygenase-1 Gene Promoter. Current Protocols in Toxicology. 6:9.7:9.7.1–9.7.21.
- Published Online: 1 MAY 2001
- Published Print: NOV 2000
Basal and inducer-dependent transcription activity of the heme oxygenase-1 gene promoter can be measured using the luciferase reporter gene in transfection assays. Methods are provided in this unit for purification of plasmid DNA, transient and stable transfection of DNA into cells, and measurement of luciferase and beta-galactosidase enzyme activities using chemiluminescent-based assays. These protocols can be used to identify inducer-activated cis elements.