Unit

UNIT 9.7 Functional Analysis of the Heme Oxygenase-1 Gene Promoter

  1. Jawed Alam

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.tx0907s06

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Alam, J. 2001. Functional Analysis of the Heme Oxygenase-1 Gene Promoter. Current Protocols in Toxicology. 6:9.7:9.7.1–9.7.21.

Author Information

  1. Alton Ochsner Medical Foundation, New Orleans, Louisiana

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: NOV 2000

Abstract

Basal and inducer-dependent transcription activity of the heme oxygenase-1 gene promoter can be measured using the luciferase reporter gene in transfection assays. Methods are provided in this unit for purification of plasmid DNA, transient and stable transfection of DNA into cells, and measurement of luciferase and beta-galactosidase enzyme activities using chemiluminescent-based assays. These protocols can be used to identify inducer-activated cis elements.