UNIT 9.9 Purification and Characterization of Heme Oxygenase

  1. Angela Wilks

Published Online: 1 MAY 2003

DOI: 10.1002/0471140856.tx0909s15

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Wilks, A. 2003. Purification and Characterization of Heme Oxygenase. Current Protocols in Toxicology. 15:9.9:9.9.1–9.9.17.

Author Information

  1. University of Maryland, Baltimore, Maryland

Publication History

  1. Published Online: 1 MAY 2003
  2. Published Print: FEB 2003


High-yield expression and purification of human heme oxygenase isozyme 1 provided the breakthrough in characterizing the protein from mechanistic and structural standpoints. This unit provides a protocol for high-level expression and subsequent purification of HO-1. The commentary includes a discussion of subsequent biochemical and biophysical characterizations.