UNIT 9.9 Purification and Characterization of Heme Oxygenase
Published Online: 1 MAY 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Wilks, A. 2003. Purification and Characterization of Heme Oxygenase. Current Protocols in Toxicology. 15:9.9:9.9.1–9.9.17.
- Published Online: 1 MAY 2003
- Published Print: FEB 2003
High-yield expression and purification of human heme oxygenase isozyme 1 provided the breakthrough in characterizing the protein from mechanistic and structural standpoints. This unit provides a protocol for high-level expression and subsequent purification of HO-1. The commentary includes a discussion of subsequent biochemical and biophysical characterizations.