UNIT 10.2 Assay of Tissue Activity of Nitric Oxide Synthase

  1. Kurt Schmidt,
  2. Bernd Mayer

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.tx1002s00

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Schmidt, K. and Mayer, B. 2001. Assay of Tissue Activity of Nitric Oxide Synthase. Current Protocols in Toxicology. 00:10.2:10.2.1–10.2.13.

Author Information

  1. Karl-Franzens-Universität Graz, Graz, Austria

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAY 1999


Nitric oxide synthase activity can be assayed by a variety of methods. The simplest method to assay activity is the conversion of radiolabeled l-arginine to l-citrulline. The assay described in this unit is sensitive, simple to perform, is relatively robust, and can be performed with intact monolayer or suspension cells, or with cell or tissue extracts. The product is easily separated from the reaction by cation-exchange chromatography.