UNIT 10.3 Detection of Nitrosated Proteins
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Zhang, Y.-Y. and Loscalzo, J. 2001. Detection of Nitrosated Proteins. Current Protocols in Toxicology. 00:10.3:10.3.1–10.3.8.
- Published Online: 1 MAY 2001
- Published Print: MAY 1999
Three methods are provided in this unit to detect nitrosated proteins. The Saville assay is the most commonly used and has the advantages that it is less sensitive to interference than the quinine sulfate-based fluorescent assay, uses simple chemical reagents, and requires a spectrophotometer. The diaminonaphthalene assay (a fluorescence assay) and chemiluminescence assay are used when the experiment requires high sensitivity. Mass spectrometry is employed for precise structural determinations.