UNIT 10.3 Detection of Nitrosated Proteins

  1. Ying-Yi Zhang,
  2. Joseph Loscalzo

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.tx1003s00

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Zhang, Y.-Y. and Loscalzo, J. 2001. Detection of Nitrosated Proteins. Current Protocols in Toxicology. 00:10.3:10.3.1–10.3.8.

Author Information

  1. Boston University School of Medicine, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAY 1999


Three methods are provided in this unit to detect nitrosated proteins. The Saville assay is the most commonly used and has the advantages that it is less sensitive to interference than the quinine sulfate-based fluorescent assay, uses simple chemical reagents, and requires a spectrophotometer. The diaminonaphthalene assay (a fluorescence assay) and chemiluminescence assay are used when the experiment requires high sensitivity. Mass spectrometry is employed for precise structural determinations.