UNIT 10.5 Measurement of cGMP and Soluble Guanylyl Cyclase Activity
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Southam, E. 2001. Measurement of cGMP and Soluble Guanylyl Cyclase Activity. Current Protocols in Toxicology. 00:10.5:10.5.1–10.5.8.
- Published Online: 1 MAY 2001
- Published Print: MAY 1999
Soluble guanylyl cyclase is responsible for synthesis of guanosine 3'5'-cyclic monophosphate. The enzyme is activated by NO, so measurement of tissue levels of cGMP and the activity of guanylyl cyclase is important in studies of NO-cGMP signal transduction. The method of choice for measuring tissue levels of cGMP is a radioimmunoassay, but it can also be measured using a scintillation proximity assay. Guanylyl cyclase activity is determined by measuring the conversion of GTP to cGMP using a radioimmunoassay or by following the conversion of [32P]GTP to [32P]cGMP, with separation of the product by precipitation and chromatography on an alumina column.