Unit

UNIT 10.6 Histochemical Analysis of Nitric Oxide Synthase by NADPH Diaphorase Staining

  1. Mirella Gonzalez-Zulueta,
  2. Valina L. Dawson,
  3. Ted M. Dawson

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.tx1006s01

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Gonzalez-Zulueta, M., Dawson, V. L. and Dawson, T. M. 2001. Histochemical Analysis of Nitric Oxide Synthase by NADPH Diaphorase Staining. Current Protocols in Toxicology. 1:10.6:10.6.1–10.6.4.

Author Information

  1. Johns Hopkins University School of Medicine, Baltimore, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: AUG 1999

Abstract

The presence of NOS coincides with that of NADPH diaphorase activity; therefore, NADPH diaphorase staining is widely used to detect NOS-containing cells in neural and non-neural tissues. With the appropriate fixation procedure, this method is capable of detecting cells containing any of the NOS isoforms.