UNIT 10.6 Histochemical Analysis of Nitric Oxide Synthase by NADPH Diaphorase Staining
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Gonzalez-Zulueta, M., Dawson, V. L. and Dawson, T. M. 2001. Histochemical Analysis of Nitric Oxide Synthase by NADPH Diaphorase Staining. Current Protocols in Toxicology. 1:10.6:10.6.1–10.6.4.
- Published Online: 1 MAY 2001
- Published Print: AUG 1999
The presence of NOS coincides with that of NADPH diaphorase activity; therefore, NADPH diaphorase staining is widely used to detect NOS-containing cells in neural and non-neural tissues. With the appropriate fixation procedure, this method is capable of detecting cells containing any of the NOS isoforms.