Unit

UNIT 10.8 Methods for Distinguishing Nitrosative and Oxidative Chemistry of Reactive Nitrogen Oxide Species Derived from Nitric Oxide

  1. David A. Wink1,
  2. Sungmee Kim1,
  3. Allen Miles2,
  4. David Jourd'heuil2,
  5. Matthew B. Grisham2

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.tx1008s03

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Wink, D. A., Kim, S., Miles, A., Jourd'heuil, D. and Grisham, M. B. 2001. Methods for Distinguishing Nitrosative and Oxidative Chemistry of Reactive Nitrogen Oxide Species Derived from Nitric Oxide. Current Protocols in Toxicology. 3:10.8:10.8.1–10.8.12.

Author Information

  1. 1

    National Cancer Institute, Bethesda, Maryland

  2. 2

    Louisiana State University Medical Center, Shreveport, Louisiana

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2000

Abstract

NO-derived intermediates formed under aerobic conditions may engage in complex chemical reactions with biologically important molecules. The outcomes of these reactions and their ultimate effect on biological systems depend on the selectivity of the species and the concentrations of different substances present and whether the reaction takes place in the gas or aqueous phase. In this unit conversion of two different compounds to fluorescent products is used to distinguish between oxidative and nitrosative chemistry of different reactive nitrogen oxide species.