UNIT 11.12 Whole-Cell Patch-Clamp Electrophysiology of Voltage-Sensitive Channels
Published Online: 1 NOV 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Shafer, T. J. 2003. Whole-Cell Patch-Clamp Electrophysiology of Voltage-Sensitive Channels. Current Protocols in Toxicology. 17:11.12:11.12.1–11.12.14.
- Published Online: 1 NOV 2003
- Published Print: AUG 2003
Ion channels are critical modulators of electrical excitability in neurons and are targets of a wide variety of neurotoxicants, including metals and insecticides. The only way to directly examine effects of toxicants on ion channel function in an individual cell on a physiologically relevant time scale is to utilize patch-clamp electrophysiological techniques. This unit presents the basics of utilizing whole-cell patch-clamp techniques to study effects of toxicants on voltage-sensitive Ca2+ channels in neurons grown in culture.
- Patch clamp;
- ion channels;