UNIT 11.12 Whole-Cell Patch-Clamp Electrophysiology of Voltage-Sensitive Channels

  1. Timothy J. Shafer

Published Online: 1 NOV 2003

DOI: 10.1002/0471140856.tx1112s17

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Shafer, T. J. 2003. Whole-Cell Patch-Clamp Electrophysiology of Voltage-Sensitive Channels. Current Protocols in Toxicology. 17:11.12:11.12.1–11.12.14.

Author Information

  1. U.S. Environmental Protection Agency, Research Triangle Park, North Carolina

Publication History

  1. Published Online: 1 NOV 2003
  2. Published Print: AUG 2003


Ion channels are critical modulators of electrical excitability in neurons and are targets of a wide variety of neurotoxicants, including metals and insecticides. The only way to directly examine effects of toxicants on ion channel function in an individual cell on a physiologically relevant time scale is to utilize patch-clamp electrophysiological techniques. This unit presents the basics of utilizing whole-cell patch-clamp techniques to study effects of toxicants on voltage-sensitive Ca2+ channels in neurons grown in culture.


  • Patch clamp;
  • electrophysiology;
  • ion channels;
  • neurotoxicants;
  • pesticides;
  • metals