UNIT 12.3 Culturing Rat Hippocampal Neurons

  1. Gerald Audesirk,
  2. Teresa Audesirk,
  3. Charles Ferguson

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.tx1203s04

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Audesirk, G., Audesirk, T. and Ferguson, C. 2001. Culturing Rat Hippocampal Neurons. Current Protocols in Toxicology. 4:12.3:12.3.1–12.3.17.

Author Information

  1. University of Colorado at Denver, Denver, Colorado

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAY 2000


Cultured neurons are widely used to investigate the mechanisms of neurotoxicity. Embryonic rat hippocampal neurons may be grown as described under a wide variety of conditions to suit differing experimental procedures, including electrophysiology, morphological analysis of neurite development, and various biochemical and molecular analyses.