UNIT 12.4 Isolation of Neonatal Rat Cortical Astrocytes for Primary Cultures
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Allen, J. W., Mutkus, L. A. and Aschner, M. 2001. Isolation of Neonatal Rat Cortical Astrocytes for Primary Cultures. Current Protocols in Toxicology. 4:12.4:12.4.1–12.4.15.
- Published Online: 1 MAY 2001
- Published Print: MAY 2000
There is increasing interest in the role of astrocytes as mediators of neurotoxicity. This unit describes a method for preparing astrocyte cultures of greater than 95% purity by enzymatic dissociation from neonatal rat brain. These preparations have both high yield and high viability.