Unit

UNIT 12.4 Isolation of Neonatal Rat Cortical Astrocytes for Primary Cultures

  1. Jeffrey W. Allen,
  2. Lysette A. Mutkus,
  3. Michael Aschner

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.tx1204s04

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Allen, J. W., Mutkus, L. A. and Aschner, M. 2001. Isolation of Neonatal Rat Cortical Astrocytes for Primary Cultures. Current Protocols in Toxicology. 4:12.4:12.4.1–12.4.15.

Author Information

  1. Wake Forest University School of Medicine, Winston-Salem, North Carolina

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAY 2000

Abstract

There is increasing interest in the role of astrocytes as mediators of neurotoxicity. This unit describes a method for preparing astrocyte cultures of greater than 95% purity by enzymatic dissociation from neonatal rat brain. These preparations have both high yield and high viability.