Unit

UNIT 12.6 Estimating Cell Number in the Central Nervous System by Stereological Methods: The Optical Disector and Fractionator

  1. Jay S. Charleston

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.tx1206s06

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Charleston, J. S. 2001. Estimating Cell Number in the Central Nervous System by Stereological Methods: The Optical Disector and Fractionator. Current Protocols in Toxicology. 6:12.6:12.6.1–12.6.19.

Author Information

  1. Institute of Neurotoxicology and Neurological Disorders and Shin Nippon Biological Laboratories U.S.A., Redmond, Washington

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: NOV 2000

Abstract

This unit describes techniques, based on recent advances in stereological methods, to obtain unbiased estimates of total cell or synapse number in discrete structures of the central nervous system. They combine unbiased counting frames, unbiased systematic random sampling, and unbiased estimates of the structure volume to produce the final estimate of number.