UNIT 12.6 Estimating Cell Number in the Central Nervous System by Stereological Methods: The Optical Disector and Fractionator
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Charleston, J. S. 2001. Estimating Cell Number in the Central Nervous System by Stereological Methods: The Optical Disector and Fractionator. Current Protocols in Toxicology. 6:12.6:12.6.1–12.6.19.
- Published Online: 1 MAY 2001
- Published Print: NOV 2000
This unit describes techniques, based on recent advances in stereological methods, to obtain unbiased estimates of total cell or synapse number in discrete structures of the central nervous system. They combine unbiased counting frames, unbiased systematic random sampling, and unbiased estimates of the structure volume to produce the final estimate of number.