UNIT 12.8 Measurement of Glial Fibrillary Acidic Protein

  1. James P. O'Callaghan

Published Online: 1 MAY 2002

DOI: 10.1002/0471140856.tx1208s11

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

O'Callaghan, J. P. 2002. Measurement of Glial Fibrillary Acidic Protein. Current Protocols in Toxicology. 11:12.8:12.8.1–12.8.12.

Author Information

  1. Center for Disease Control and Prevention NIOSH, Morgantown, West Virginia

Publication History

  1. Published Online: 1 MAY 2002
  2. Published Print: FEB 2002


Toxicant-induced injury of the adult or developing central nervous system results in hypertrophy of astrocytes at the site of injury. The hallmark of this response is enhanced expression of the major intermediate filament protein of astrocytes, glial fibrillary acid protein (GFAP). A protocol is provided for assaying GFAP levels in detergent homogenates of brain tissue using a sandwich ELISA.