UNIT 12.8 Measurement of Glial Fibrillary Acidic Protein
Published Online: 1 MAY 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
O'Callaghan, J. P. 2002. Measurement of Glial Fibrillary Acidic Protein. Current Protocols in Toxicology. 11:12.8:12.8.1–12.8.12.
- Published Online: 1 MAY 2002
- Published Print: FEB 2002
Toxicant-induced injury of the adult or developing central nervous system results in hypertrophy of astrocytes at the site of injury. The hallmark of this response is enhanced expression of the major intermediate filament protein of astrocytes, glial fibrillary acid protein (GFAP). A protocol is provided for assaying GFAP levels in detergent homogenates of brain tissue using a sandwich ELISA.