UNIT 12.9 Aggregating Neural Cell Cultures

  1. Paul Honegger

Published Online: 1 MAY 2003

DOI: 10.1002/0471140856.tx1209s15

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Honegger, P. 2003. Aggregating Neural Cell Cultures. Current Protocols in Toxicology. 15:12.9:12.9.1–12.9.17.

Author Information

  1. University of Lausanne, Lausanne, Switzerland

Publication History

  1. Published Online: 1 MAY 2003
  2. Published Print: FEB 2003


Aggregating Neural Cell Cultures (Paul Honegger, University of Lausanne, Lausanne, Switzerland). When freshly dissociated embryonic tissues are kept under gyratory agitation, the cells aggregate to form three-dimensional spheroids in which the cells can migrate and organize themselves, attaining maximal cellular differentiation after weeks of culture. The three-dimensional architecture of the aggregates permits direct cell-to-cell interactions and the formation of a natural cell matrix, which is fundamental to the acquisition of the histotypic properties of the aggregates. This unit describes protocols for preparing forebrain cells from embryonic rodents for aggregating cultures and maintaining these cultures to the differentiated state.