UNIT 12.9 Aggregating Neural Cell Cultures
Published Online: 1 MAY 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Honegger, P. 2003. Aggregating Neural Cell Cultures. Current Protocols in Toxicology. 15:12.9:12.9.1–12.9.17.
- Published Online: 1 MAY 2003
- Published Print: FEB 2003
Aggregating Neural Cell Cultures (Paul Honegger, University of Lausanne, Lausanne, Switzerland). When freshly dissociated embryonic tissues are kept under gyratory agitation, the cells aggregate to form three-dimensional spheroids in which the cells can migrate and organize themselves, attaining maximal cellular differentiation after weeks of culture. The three-dimensional architecture of the aggregates permits direct cell-to-cell interactions and the formation of a natural cell matrix, which is fundamental to the acquisition of the histotypic properties of the aggregates. This unit describes protocols for preparing forebrain cells from embryonic rodents for aggregating cultures and maintaining these cultures to the differentiated state.