UNIT 12.15 Application of Single-Cell Microfluorimetry to Neurotoxicology Assays
Published Online: 1 NOV 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Limke, T. L. and Atchison, W. D. 2009. Application of Single-Cell Microfluorimetry to Neurotoxicology Assays. Current Protocols in Toxicology. 42:12.15:12.15.1–12.15.13.
- Published Online: 1 NOV 2009
- Published Print: NOV 2009
Intracellular signaling events play fundamental roles in regulating physiological function. In neurons, these include inducing growth and differentiation, secretion, gene expression, and controlling processes associated with learning and memory. All of these processes have in common the vital dependence on changes in intracellular Ca2+ [Ca2+]i. Numerous toxicants, including metals, polychlorinated biphenyls, and biological neurotoxins, can disrupt [Ca2+]i. Understanding how toxicants disrupt Ca2+-dependent neuronal signaling, and thus induce neuronal death or dysfunction, requires the ability to monitor [Ca2+]i at the level of individual cells. A series of fluorophores that can report on changes in [Ca2+]i has been pivotal in this process. This section describes how to use these fluorophores to study effects of neurotoxicants on two types of processes: changes in [Ca2+]i in individual cells and changes in mitochondrial membrane potential. Similar techniques using distinct fluorophores can be applied to other physiological processes. Curr. Protoc. Toxicol. 42:12.15.1-12.15.13. © 2009 by John Wiley & Sons, Inc.