UNIT 12.20 Assessment of Adult Neurogenesis in Mice
Published Online: 1 MAY 2013
Copyright © 2013 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Pan, Y.-W., Wang, W. and Xia, Z. 2013. Assessment of Adult Neurogenesis in Mice. Current Protocols in Toxicology. 56:12.20:12.20.1–12.20.16.
- Published Online: 1 MAY 2013
- Published Print: MAY 2013
Adult neurogenesis is a lifelong developmental process that occurs in two discrete regions in the adult mammalian brain: the subgranular zone of the dentate gyrus (DG) and the subventricular zone (SVZ) along the lateral ventricles. Despite immense interest in the therapeutic potential of adult neural stem cells (aNSCs) residing along these two neurogenic regions, molecular and cellular mechanisms regulating this process are not fully defined. Defining the regulatory mechanisms responsible for the genesis of new neurons in the adult brain is integral to understanding the basic biology of aNSCs. The techniques described here provide a basic blueprint to isolate, culture, and perform experiments using aNSCs in vitro as well as providing methods to perform immunohistochemistry on brain sections. Curr. Protoc. Toxicol. 56:12.20.1-12.20.16. © 2013 by John Wiley & Sons, Inc.
- adult neural stem cells (aNSC);
- primary culture;
- primary cell isolation;
- brain section