UNIT 13.2 Rat Embryo Cultures for In Vitro Teratology
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Fantel, A. G., Bechter, R. and Beckman, D. 2001. Rat Embryo Cultures for In Vitro Teratology. Current Protocols in Toxicology. 6:13.2:13.2.1–13.2.12.
- Published Online: 1 MAY 2001
- Published Print: NOV 2000
Mammalian embryos provide a particular challenge to those who wish to study developmental processes because development takes place inside the mother's body, thus limiting the investigator's ability to directly affect and observe the embryonic stages of development. Fortunately, as this unit illustrates, methods have been developed for in vitro culture of rodent embryos during early postimplantation (gestation days 9 to 11) and early fetal (gestation days 12 to 14) stages, which are the periods during which the major systems of the embryo are established. Embryos at these stages are particularly suitable for screening studies to determine the effects of teratological agents.