UNIT 13.2 Rat Embryo Cultures for In Vitro Teratology

  1. Alan G. Fantel1,
  2. Rudolf Bechter2,
  3. David Beckman3

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.tx1302s06

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Fantel, A. G., Bechter, R. and Beckman, D. 2001. Rat Embryo Cultures for In Vitro Teratology. Current Protocols in Toxicology. 6:13.2:13.2.1–13.2.12.

Author Information

  1. 1

    University of Washington, Seattle, Washington

  2. 2

    Novartis Pharma Ltd., Basel, Switzerland

  3. 3

    Novartis Pharmaceuticals Corporation, East Hanover, New Jersey

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: NOV 2000


Mammalian embryos provide a particular challenge to those who wish to study developmental processes because development takes place inside the mother's body, thus limiting the investigator's ability to directly affect and observe the embryonic stages of development. Fortunately, as this unit illustrates, methods have been developed for in vitro culture of rodent embryos during early postimplantation (gestation days 9 to 11) and early fetal (gestation days 12 to 14) stages, which are the periods during which the major systems of the embryo are established. Embryos at these stages are particularly suitable for screening studies to determine the effects of teratological agents.