UNIT 13.3 Micromass Cultures in Teratology
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Ponce, R. A. 2001. Micromass Cultures in Teratology. Current Protocols in Toxicology. 7:13.3:13.3.1–13.3.14.
- Published Online: 1 MAY 2001
- Published Print: FEB 2001
This unit describes methods for culture of undifferentiated midbrain (mesencephalon) and limb bud cells from gestation day 12 rat embryos. When grown over 5 days in vitro, these mixed cell populations express many morphological, biochemical, molecular, and immunophenotypic characteristics observed during in vivo differentiation. These cultures can be used in a wide variety of studies designed to investigate normal cellular ontogeny, the teratogenic potential of test agents, or the mechanisms underlying the cellular response to environmental stress.