UNIT 13.3 Micromass Cultures in Teratology

  1. Rafael A. Ponce

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.tx1303s07

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Ponce, R. A. 2001. Micromass Cultures in Teratology. Current Protocols in Toxicology. 7:13.3:13.3.1–13.3.14.

Author Information

  1. University of Washington, Seattle, Washington

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2001


This unit describes methods for culture of undifferentiated midbrain (mesencephalon) and limb bud cells from gestation day 12 rat embryos. When grown over 5 days in vitro, these mixed cell populations express many morphological, biochemical, molecular, and immunophenotypic characteristics observed during in vivo differentiation. These cultures can be used in a wide variety of studies designed to investigate normal cellular ontogeny, the teratogenic potential of test agents, or the mechanisms underlying the cellular response to environmental stress.