UNIT 13.6 Organ Culture of Midfacial Tissue and Secondary Palate
Published Online: 1 AUG 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Abbott, B. D. 2003. Organ Culture of Midfacial Tissue and Secondary Palate. Current Protocols in Toxicology. 16:13.6:13.6.1–13.6.11.
- Published Online: 1 AUG 2003
- Published Print: MAY 2003
Palatal organ culture provides an in vitro model for the study of the formation of the secondary palate, which forms the roof of the mouth in the developing fetus. The protocol describes the steps for culture of the mid-facial region of the fetal mouse or rat. In culture the secondary palatal shelves proceed through stages of growth, elevation and fusion in a manner analogous to that occurring in utero. This model provides a tool for studies of mechanisms of normal and abnormal palatogenesis and has applications for developmental biology and toxicology.
- cleft palate;
- palate organ culture