UNIT 14.3 Small Animal Models of Hemorrhagic Shock–Induced Liver Dysfunction
Published Online: 1 MAY 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Bauer, I. and Bauer, M. 2002. Small Animal Models of Hemorrhagic Shock–Induced Liver Dysfunction. Current Protocols in Toxicology. 11:14.3:14.3.1–14.3.8.
- Published Online: 1 MAY 2002
- Published Print: FEB 2002
Blood loss and ensuing circulatory failure continues to be the leading cause of death between the ages of 1 and 45 in most western countries. Hepatocellular dysfunction is a key feature of the pathophysiological sequelae of trauma/hemorrhage in patients surviving the first hours and hemorrhagic hypotension has been widely used as a model for indirect liver injury in the critically ill. This unit describes a low-flow ischemia model of the liver due to hemorrhagic hypotension that can be used to produce a reproducible and graded injury to the pericentral region of the acinus, which is critical for metabolism of xenobiotics. This protocol allows for measurement of cardiac output and regional flow.