UNIT 14.4 Isolation of Liver Kupffer Cells

  1. Matthias Froh1,
  2. Akira Konno2,
  3. Ronald G. Thurman2

Published Online: 1 FEB 2003

DOI: 10.1002/0471140856.tx1404s14

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Froh, M., Konno, A. and Thurman, R. G. 2003. Isolation of Liver Kupffer Cells. Current Protocols in Toxicology. 14:14.4:14.4.1–14.4.12.

Author Information

  1. 1

    University of Regensburg, Regensburg, Germany

  2. 2

    University of North Carolina, Chapel Hill, North Carolina

Publication History

  1. Published Online: 1 FEB 2003
  2. Published Print: NOV 2002


Isolation of Liver Kupffer Cells (Matthias Froh, Akira Konno, Ronald G. Thurman, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina). Kupffer cells, the resident macrophages of the liver, play a major role in the pathogenesis of several diseases. This unit contains an easy-to-follow procedure for effective isolation of liver Kupffer cells from rats and mice. The protocol provides viable Kupffer cells in large amounts that can be used for further investigations.