Unit

UNIT 15.1 Embryonic Stem (ES) Cell Culture Basics

  1. Jennifer V. Schmidt

Published Online: 1 NOV 2001

DOI: 10.1002/0471140856.tx1501s09

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Schmidt, J. V. 2001. Embryonic Stem (ES) Cell Culture Basics. Current Protocols in Toxicology. 9:15.1:15.1.1–15.1.15.

Author Information

  1. University of Illinois at Chicago, Chicago, Illinois

Publication History

  1. Published Online: 1 NOV 2001
  2. Published Print: AUG 2001

Abstract

This unit describes the procedures to grow and maintain embryonic stem (ES) cells in culture. Growth-inactivated mouse embryo fibroblasts (MEF) are required as a feeder layer for the ES cells; their isolation and culture is described as are the additional protocols required for generating a mouse with a particular targeted gene electroporation, cloning and selection for homologous recombinants, transfection with Cre, and preparation of cells for injection.