UNIT 15.1 Embryonic Stem (ES) Cell Culture Basics
Published Online: 1 NOV 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Schmidt, J. V. 2001. Embryonic Stem (ES) Cell Culture Basics. Current Protocols in Toxicology. 9:15.1:15.1.1–15.1.15.
- Published Online: 1 NOV 2001
- Published Print: AUG 2001
This unit describes the procedures to grow and maintain embryonic stem (ES) cells in culture. Growth-inactivated mouse embryo fibroblasts (MEF) are required as a feeder layer for the ES cells; their isolation and culture is described as are the additional protocols required for generating a mouse with a particular targeted gene electroporation, cloning and selection for homologous recombinants, transfection with Cre, and preparation of cells for injection.