UNIT 16.10 Preparation of Rodent Testis Co-Cultures
Published Online: 1 FEB 2013
Copyright © 2013 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Wegner, S., Hong, S., Yu, X. and Faustman, E. M. 2013. Preparation of Rodent Testis Co-Cultures. Current Protocols in Toxicology. 55:16.10.1–16.10.7.
- Published Online: 1 FEB 2013
Male reproductive development is a complex process that is sensitive to disruption by a range of toxicants. There is a great need for in vitro models that can evaluate potential male reproductive toxicants. The current unit presents a protocol for preparation of a three-dimensional in vitro model of male reproductive development that reduces the number of animals required for evaluation of toxicants. A Matrigel overlay provides a three-dimensional extracellular matrix that improves cell attachment, viability, and communication, and makes the model more reflective of in vivo environments. Curr. Protoc. Toxicol. 55:16.10.1-16.10.7. © 2013 by John Wiley & Sons, Inc.
- male reproductive development;
- three-dimensional in vitro models;
- primary cell culture