UNIT 17.2 Measurement of Protein Sulfenic Acid Content
Published Online: 1 NOV 2008
Copyright © 2008 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Poole, L. B. 2008. Measurement of Protein Sulfenic Acid Content. Current Protocols in Toxicology. 38:17.2:17.2.1–17.2.27.
- Published Online: 1 NOV 2008
- Published Print: NOV 2008
Protein sulfenic acids are reactive, reversibly oxidized cysteinyl residues with roles in redox catalysis and regulation. Detection and quantification of these species in proteins is accomplished through chemical modification by reagents such as 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD chloride), 2-nitro-5-thiobenzoate (TNB), dimedone, or derivatives of dimedone, followed by UV-visible spectroscopy or mass spectrometric analysis. Curr. Protoc. Toxicol. 38:17.2.1-17.2.27. © 2008 by John Wiley & Sons, Inc.
- sulfenic acids;
- cysteine modification;
- cysteine oxidation;
- UV-visible spectroscopy;
- mass spectrometry