UNIT 17.2 Measurement of Protein Sulfenic Acid Content

  1. Leslie B. Poole

Published Online: 1 NOV 2008

DOI: 10.1002/0471140856.tx1702s38

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Poole, L. B. 2008. Measurement of Protein Sulfenic Acid Content. Current Protocols in Toxicology. 38:17.2:17.2.1–17.2.27.

Author Information

  1. Wake Forest University School of Medicine, Winston-Salem, North Carolina

Publication History

  1. Published Online: 1 NOV 2008
  2. Published Print: NOV 2008


Protein sulfenic acids are reactive, reversibly oxidized cysteinyl residues with roles in redox catalysis and regulation. Detection and quantification of these species in proteins is accomplished through chemical modification by reagents such as 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD chloride), 2-nitro-5-thiobenzoate (TNB), dimedone, or derivatives of dimedone, followed by UV-visible spectroscopy or mass spectrometric analysis. Curr. Protoc. Toxicol. 38:17.2.1-17.2.27. © 2008 by John Wiley & Sons, Inc.


  • sulfenic acids;
  • cysteine modification;
  • cysteine oxidation;
  • UV-visible spectroscopy;
  • mass spectrometry