Unit

UNIT 17.3 Fluorescence Microplate Reader Measurement of Tissue Susceptibility to Lipid Peroxidation

  1. Jin-Gang Zhang1,
  2. Marc W. Fariss2

Published Online: 1 FEB 2004

DOI: 10.1002/0471140856.tx1703s18

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Zhang, J.-G. and Fariss, M. W. 2004. Fluorescence Microplate Reader Measurement of Tissue Susceptibility to Lipid Peroxidation. Current Protocols in Toxicology. 18:17.3:17.3.1–17.3.10.

Author Information

  1. 1

    BD Biosciences, Woburn, Massachusetts

  2. 2

    University of Colorado Health Sciences Center, Denver, Colorado

Publication History

  1. Published Online: 1 FEB 2004
  2. Published Print: NOV 2003

Abstract

This unit describes a method for the measurement of cellular membrane antioxidant capacity or susceptibility of tissue samples to lipid peroxidation using a fluorescence microplate reader. The assay is simple and has the advantage of monitoring susceptibility to lipid peroxidation in a large number of samples in real time.

Keywords:

  • antioxidant;
  • susceptibility to lipid peroxidation;
  • antioxidant capacity;
  • fluorescence plate reader;
  • vitamin E;
  • vitamin E succinate