UNIT 17.11 Measuring Electrophile Stress

  1. Keri A. Tallman,
  2. Andrew Vila,
  3. Ned A. Porter,
  4. Lawrence J. Marnett

Published Online: 1 MAY 2009

DOI: 10.1002/0471140856.tx1711s40

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Tallman, K. A., Vila, A., Porter, N. A. and Marnett, L. J. 2009. Measuring Electrophile Stress. Current Protocols in Toxicology. 40:17.11:17.11.1–17.11.13.

Author Information

  1. Vanderbilt University Medical Center, Nashville, Tennessee

Publication History

  1. Published Online: 1 MAY 2009
  2. Published Print: MAY 2009


Polyunsaturated fatty acids are primary targets during oxidative stress. Diffusible electrophilic α,β-unsaturated aldehydes, such as 4-hydroxynonenal (HNE), have been shown to modify proteins that mediate cell signaling and modify gene expression pathways. We describe a global strategy for identifying the protein targets of HNE modification. A similar approach can be used for any electrophiles derived from an oxidized lipid. Curr. Protoc. Toxicol. 40:17.11.1-17.11.13. © 2009 by John Wiley & Sons, Inc.


  • electrophile;
  • click chemistry;
  • protein modification