UNIT 17.11 Measuring Electrophile Stress
Published Online: 1 MAY 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Tallman, K. A., Vila, A., Porter, N. A. and Marnett, L. J. 2009. Measuring Electrophile Stress. Current Protocols in Toxicology. 40:17.11:17.11.1–17.11.13.
- Published Online: 1 MAY 2009
- Published Print: MAY 2009
Polyunsaturated fatty acids are primary targets during oxidative stress. Diffusible electrophilic α,β-unsaturated aldehydes, such as 4-hydroxynonenal (HNE), have been shown to modify proteins that mediate cell signaling and modify gene expression pathways. We describe a global strategy for identifying the protein targets of HNE modification. A similar approach can be used for any electrophiles derived from an oxidized lipid. Curr. Protoc. Toxicol. 40:17.11.1-17.11.13. © 2009 by John Wiley & Sons, Inc.
- click chemistry;
- protein modification