Unit

UNIT 18.8 Immune Cell Phenotyping Using Flow Cytometry

  1. Julie A. Oughton,
  2. Nancy I. Kerkvliet

Published Online: 1 MAR 2005

DOI: 10.1002/0471140856.tx1808s23

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Oughton, J. A. and Kerkvliet, N. I. 2005. Immune Cell Phenotyping Using Flow Cytometry. Current Protocols in Toxicology. 23:18.8:18.8.1–18.8.24.

Author Information

  1. Oregon State University, Corvallis, Oregon

Publication History

  1. Published Online: 1 MAR 2005
  2. Published Print: FEB 2005

Abstract

Immunophenotyping of lymphoid tissues, particularly spleen and thymus, has been used in the field of immunotoxicology for many years as part of a tiered screening approach for detecting immunotoxicity following chemical exposure. This unit describes basic procedures for direct and indirect immunofluorescent antibody staining of surface proteins on lymphoid cells from tissues or blood. Protocols for resolution of dead cells and for fixation of cells are also included. In addition, information on antibody selection and titration, fluorochrome choices, isotype controls, compensation, standardization of flow cytometric analysis, data analysis, and statistical issues is presented.